Application
Fluorescent image of U251 cells stained with LAMP3 (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1827a LAMP3 (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LAMP3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.Application
LAMP3 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the LAMP3 antibody detected the LAMP3 protein (arrow).| Product Name | LAMP3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LAMP3; DCLAMP; TSC403; Lysosome-associated membrane glycoprotein 3; DC-lysosome-associated membrane glycoprotein; Protein TSC403; CD_antigen=CD208; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| IF:1:100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DCLAMP TSC403 |
|---|---|
| Alternative Names | LAMP3 DCLAMP TSC403 Lysosome-associated membrane glycoprotein 3 DC-lysosome-associated membrane glycoprotein Protein TSC403 CD_antigen=CD208 Flags: Precursor |
| Molecular Weight(MW) | 44346 Da |
| Function | May play a role in dendritic cell function and in adaptive immunity |
| Tissue Specificity | Detected in tonsil interdigitating dendritic cells, in spleen, lymph node, Peyer's patches in the small instestine, in thymus medulla and in B cells (at protein level) Expressed in lymphoid organs and dendritic cells. Expressed in lung. Up-regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues |
| Cellular Localization | Lysosome membrane; Single-pass type I membrane protein. Cytoplasmic vesicle membrane; Single-pass type I membrane protein. Note=During dendritic cell maturation, detected on cytoplasmic vesicles (the MHC II compartment) that contain MHC II proteins, LAMP1, LAMP2 and LAMP3. Detected on lysosomes in mature dendritic cells |
| Entrez Gene | 27074 |
|---|
Application
Fluorescent image of U251 cells stained with LAMP3 (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1827a LAMP3 (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LAMP3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Application
LAMP3 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the LAMP3 antibody detected the LAMP3 protein (arrow).| Application Notes | IF:1:100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
