Application
Fluorescent image of U251 cells stained with LAMP1 (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1823a LAMP1 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LAMP1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.Application
Western blot analysis of LAMP1 Antibody (N-term) in Y79 cell line lysates (35ug/lane). LAMP1 (arrow) was detected using the purified Pab (1:60 dilution).Application
Western blot analysis of LAMP1 Antibody (N-term) in mouse lung tissue lysates (35ug/lane).LAMP1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *LAMP1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | LAMP1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LAMP1; Lysosome-associated membrane glycoprotein 1; CD107 antigen-like family member A; CD_antigen=CD107a; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWBIHC |
| IF:1:200 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | LAMP1 Lysosome-associated membrane glycoprotein 1 CD107 antigen-like family member A CD_antigen=CD107a Flags: Precursor |
|---|---|
| Molecular Weight(MW) | 44882 Da |
| Function | Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis |
| Tissue Specificity | This LAMP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131~161 amino acids from the N-terminal region of human LAMP1. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein Lysosome membrane; Single-pass type I membrane protein. Late endosome. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane. Co-localizes with OSBPL1A at the late endosome |
| Entrez Gene | 3916 |
|---|
Application
Fluorescent image of U251 cells stained with LAMP1 (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1823a LAMP1 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LAMP1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Application
Western blot analysis of LAMP1 Antibody (N-term) in Y79 cell line lysates (35ug/lane). LAMP1 (arrow) was detected using the purified Pab (1:60 dilution).
Application
Western blot analysis of LAMP1 Antibody (N-term) in mouse lung tissue lysates (35ug/lane).LAMP1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *LAMP1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | IF:1:200 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
