Application
Western blot analysis of LAT Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). LAT (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human breast carcinoma with LAT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | LAT Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LAT; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; p36-38 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | LAT Linker for activation of T-cells family member 1 36 kDa phospho-tyrosine adapter protein p36-38 |
|---|---|
| Molecular Weight(MW) | 27930 Da |
| Function | Required for TCR (T-cell antigen receptor)- and pre-TCR- mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules |
| Tissue Specificity | Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level) |
| Cellular Localization | Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts |
| Entrez Gene | 27040 |
|---|
Application
Western blot analysis of LAT Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). LAT (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma with LAT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
