Application
A. Western blot analysis on Jurkat cell lysate using anti-Phospho-LAT (pY191) RabMAb (Cat. #AJ1434b); dilution 1:2,000. (1) Jurkat plus anti-CDC and (2) Jurkat negative control.Application
B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-Phospho-LAT (pY191) RabMAb (Cat. #AJ1434b).| Product Name | LAT Antibody Phospho (pY191) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LAT, Linker for activation of T-cells family member 1, 36 kDa phospho-tyrosine adapter protein;pp36;p36-38 |
| Modification | p-Y191 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E225 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | LAT Linker for activation of T-cells family member 1 36 kDa phospho-tyrosine adapter protein pp36 p36-38 |
|---|---|
| Molecular Weight(MW) | 27930 Da |
| Function | Required for TCR (T-cell antigen receptor)- and pre-TCR- mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules |
| Tissue Specificity | Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level) |
| Cellular Localization | Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts |
| Entrez Gene | 27040 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-Phospho-LAT (pY191) RabMAb (Cat. #AJ1434b); dilution 1:2,000. (1) Jurkat plus anti-CDC and (2) Jurkat negative control.
Application
B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-Phospho-LAT (pY191) RabMAb (Cat. #AJ1434b).| Application Notes | WB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
