Application
Western blot analysis of Phospho-APG8a (MAP1LC3A)-S12 in HepG2 cell line lysates (35ug/lane). MAP1LC3A (arrow) was detected using the purified Pab.Application
Western blot analysis of anti-APG8a (MAP1LC3A) Antibody (S12) in mouse cerebellum tissue lysates (35ug/lane). MAP1LC3A (arrow) was detected using the purified Pab.Application
Immunofluorescence analysis of APG8a (MAP1LC3A) Antibody (S12) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.| Product Name | LC3 Antibody (APG8A) (S12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP1LC3A; Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; Microtubule-associated protein 1 light chain 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MAP1LC3A Microtubule-associated proteins 1A/1B light chain 3A Autophagy-related protein LC3 A Autophagy-related ubiquitin-like modifier LC3 A MAP1 light chain 3-like protein 1 MAP1A/MAP1B light chain 3 A Microtubule-associated protein 1 light chain 3 |
|---|---|
| Molecular Weight(MW) | 14272 Da |
| Function | Involved in formation of autophagosomal vacuoles (autophagosomes) |
| Tissue Specificity | Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes |
| Cellular Localization | Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome. Note=LC3-II binds to the autophagic membranes |
| Entrez Gene | 84557 |
|---|
Application
Western blot analysis of Phospho-APG8a (MAP1LC3A)-S12 in HepG2 cell line lysates (35ug/lane). MAP1LC3A (arrow) was detected using the purified Pab.
Application
Western blot analysis of anti-APG8a (MAP1LC3A) Antibody (S12) in mouse cerebellum tissue lysates (35ug/lane). MAP1LC3A (arrow) was detected using the purified Pab.
Application
Immunofluorescence analysis of APG8a (MAP1LC3A) Antibody (S12) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
