Application
The anti-LC3 (APG8c) Pab is used in Western blot to detect LC3 (APG8c) in, from left to right, 293, Ramos, Hela, HepG2, T47d, and Jurkat tissue lysates. LC3 (APG8c)(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | LC3 Antibody (APG8C) (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP1LC3C; Microtubule-associated proteins 1A/1B light chain 3C; Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; MAP1 light chain 3-like protein 3; MAP1A/MAP1B light chain 3 C; Microtubule-associated protein 1 light chain 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MAP1LC3C Microtubule-associated proteins 1A/1B light chain 3C Autophagy-related protein LC3 C Autophagy-related ubiquitin-like modifier LC3 C MAP1 light chain 3-like protein 3 MAP1A/MAP1B light chain 3 C Microtubule-associated protein 1 light chain 3 |
|---|---|
| Molecular Weight(MW) | 16852 Da |
| Function | Probably involved in formation of autophagosomal vacuoles (autophagosomes) (By similarity) |
| Tissue Specificity | Most abundant in placenta, lung and ovary. |
| Cellular Localization | Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes |
| Entrez Gene | 440738 |
|---|
Application
The anti-LC3 (APG8c) Pab is used in Western blot to detect LC3 (APG8c) in, from left to right, 293, Ramos, Hela, HepG2, T47d, and Jurkat tissue lysates. LC3 (APG8c)(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
