Application
Lcn2 Antibody (Human N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Lcn2 antibody detected the Lcn2 protein (arrow).| Product Name | LCN2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LCN2; HNL; NGAL; Neutrophil gelatinase-associated lipocalin; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNL NGAL |
|---|---|
| Alternative Names | LCN2 HNL NGAL Neutrophil gelatinase-associated lipocalin 25 kDa alpha-2-microglobulin-related subunit of MMP-9 Lipocalin-2 Oncogene 24p3 Siderocalin LCN2 p25 |
| Molecular Weight(MW) | 22588 Da |
| Function | Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth |
| Tissue Specificity | Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes |
| Cellular Localization | Secreted. Note=Upon binding to the SLC22A17 (24p3R) receptor, it is internalized |
| Entrez Gene | 3934 |
|---|
Application
Lcn2 Antibody (Human N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Lcn2 antibody detected the Lcn2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
