Application
A. Western blot analysis on HepG2 cell lysate using anti-LDL receptor RabMAb (Cat. #AJ1440a), dilution 1:5000.Application
B. Immunohistochemical staining of paraffin-embedded human liver using anti-LDLR RabMAb (Cat. #AJ1440a).Application
C. Immunofluorescent staining of HEPG2 cells using anti-LDLR RabMAb (Cat. #AJ1440a).| Product Name | LDL Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LDLR, Low-density lipoprotein receptor |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1553Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:10000~50000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | LDLR Low-density lipoprotein receptor |
|---|---|
| Molecular Weight(MW) | 95376 Da |
| Function | Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells |
| Tissue Specificity | A synthetic peptide corresponding to residues on the C-terminus of human LDL receptor was used as an immunogen. This antibody detects both isoforms of LDL receptor. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Endomembrane system; Single-pass type I membrane protein Membrane, clathrin-coated pit; Single-pass type I membrane protein. Golgi apparatus. Early endosome. Late endosome. Cell surface. Lysosome. Note=Found distributed from the plasma membrane to intracellular compartments. Localizes to the Golgi apparatus, early and late endosomes/lysosomes and cell surface in the presence of PCSK9 |
| Entrez Gene | 3949 |
|---|
Application
A. Western blot analysis on HepG2 cell lysate using anti-LDL receptor RabMAb (Cat. #AJ1440a), dilution 1:5000.
Application
B. Immunohistochemical staining of paraffin-embedded human liver using anti-LDLR RabMAb (Cat. #AJ1440a).
Application
C. Immunofluorescent staining of HEPG2 cells using anti-LDLR RabMAb (Cat. #AJ1440a).| Application Notes | WB:1:10000~50000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
