Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with LECT1 antibody (C-term) (Cat.#Ap2729b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of anti-LECT1 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane).LECT1(arrow) was detected using the purified Pab.Application
Western blot analysis of LECT1 (arrow) using rabbit polyclonal LECT1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LECT1 gene.| Product Name | LECT1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LECT1; CHMI; Leukocyte cell-derived chemotaxin 1; Chondrosurfactant protein; Chondromodulin-1; Chondromodulin-I |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CHMI |
|---|---|
| Alternative Names | LECT1 CHMI Leukocyte cell-derived chemotaxin 1 Chondrosurfactant protein Chondromodulin-1 Chondromodulin-I |
| Molecular Weight(MW) | 37102 Da |
| Function | Bifunctional growth regulator that stimulates the growth of cultured chondrocytes in the presence of basic fibroblast growth factor (FGF) but inhibits the growth of cultured vascular endothelial cells. May contribute to the rapid growth of cartilage and vascular invasion prior to the replacement of cartilage by bone during endochondral bone development. Inhibits in vitro tube formation and mobilization of endothelial cells. Plays a role as antiangiogenic factor in cardiac valves to suppress neovascularization |
| Tissue Specificity | Detected in cartilage and cardiac valves (at protein level). Detected in the laminae fibrosa, spongiosa and ventricularis layers of normal cardiac valves (at protein level) Expression is decreased cardiac valves of patients with valvular heart disease (at protein level). Weakly expressed in chondrosarcoma |
| Cellular Localization | Chondromodulin-1: Secreted. Note=Accumulated in the inter-territorial matrix of cartilage |
| Entrez Gene | 11061 |
|---|
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with LECT1 antibody (C-term) (Cat.#Ap2729b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of anti-LECT1 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane).LECT1(arrow) was detected using the purified Pab.
Application
Western blot analysis of LECT1 (arrow) using rabbit polyclonal LECT1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LECT1 gene.| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
