Application
Western blot analysis of hLep-R149 in MDA-MB231 cell line lysates (35ug/lane). LEP (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of Leptin (LEP) Antibody (C-term)(Cat#AP6150a) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | Leptin (LEP) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LEP; OB; OBS; Leptin; Obese protein; Obesity factor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OB OBS |
|---|---|
| Alternative Names | LEP OB OBS Leptin Obese protein Obesity factor |
| Molecular Weight(MW) | 18641 Da |
| Function | May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass |
| Tissue Specificity | This LEP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138~167 amino acids from the C-terminal region of human LEP. |
| Cellular Localization | Secreted. |
Application
Western blot analysis of hLep-R149 in MDA-MB231 cell line lysates (35ug/lane). LEP (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of Leptin (LEP) Antibody (C-term)(Cat#AP6150a) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
