Application
LEP Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the LEP antibody detected the LEP protein (arrow).Application
Western blot analysis of LEP (arrow) using rabbit polyclonal LEP Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LEP gene.Application
Confocal immunofluorescent analysis of LEP Antibody (N-term)(Cat#AP16666a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
LEP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LEP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
LEP Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | LEP Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LEP; OB; OBS; Leptin; Obese protein; Obesity factor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFIHCFC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OB OBS |
|---|---|
| Alternative Names | LEP OB OBS Leptin Obese protein Obesity factor |
| Molecular Weight(MW) | 18641 Da |
| Function | May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass |
| Tissue Specificity | This LEP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-37 amino acids from the N-terminal region of human LEP. |
| Cellular Localization | Secreted. |
| Entrez Gene | 3952 |
|---|---|
| Nucleotide Accession | NP_000221.1 |
Application
LEP Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the LEP antibody detected the LEP protein (arrow).
Application
Western blot analysis of LEP (arrow) using rabbit polyclonal LEP Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LEP gene.
Application
Confocal immunofluorescent analysis of LEP Antibody (N-term)(Cat#AP16666a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
LEP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LEP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
LEP Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
