Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-LFA-beta2 (pS745) RabMAb (Cat. #AJ1443b), 1:500 dilution. Cells were either (A) untreated (B) treated with AP.Application
B. Immunohistochemical analysis of human tonsils using anti-Phospho- LFA-beta2 (pS745) RabMAb (Cat. #AJ1443b).| Product Name | LFA-beta2 (ITGB2) Antibody Phospho (pS745) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ITGB2, CD18, MFI7, Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta;Complement receptor C3 subunit beta |
| Modification | p-S745 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1288Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD18 MFI7 |
|---|---|
| Alternative Names | ITGB2 CD18 MFI7 Integrin beta-2 Cell surface adhesion glycoproteins LFA-1/CR3/p150 95 subunit beta Complement receptor C3 subunit beta |
| Molecular Weight(MW) | 84782 Da |
| Function | Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha- D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Ser745 of human LFA-β2 was used as immunogen. The antibody only detects LFA-β2 phosphorylated on Serine 745. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 3689 |
|---|
Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-LFA-beta2 (pS745) RabMAb (Cat. #AJ1443b), 1:500 dilution. Cells were either (A) untreated (B) treated with AP.
Application
B. Immunohistochemical analysis of human tonsils using anti-Phospho- LFA-beta2 (pS745) RabMAb (Cat. #AJ1443b).| Application Notes | WB:1:5000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
