Application
LFNG Antibody (Center) western blot analysis in HL-60,K562,Hela cell line lysates (35ug/lane).This demonstrates the LFNG antibody detected the LFNG protein (arrow).Application
LFNG Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LFNG Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
LFNG Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | LFNG Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LFNG; Beta-1, 3-N-acetylglucosaminyltransferase lunatic fringe; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | LFNG Beta-1 3-N-acetylglucosaminyltransferase lunatic fringe O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
|---|---|
| Molecular Weight(MW) | 41773 Da |
| Function | Glycosyltransferase that initiates the elongation of O- linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning (By similarity) |
| Tissue Specificity | This LFNG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93~122 amino acids from the Central region of human LFNG. |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein (By similarity) |
| Entrez Gene | 3955 |
|---|
Application
LFNG Antibody (Center) western blot analysis in HL-60,K562,Hela cell line lysates (35ug/lane).This demonstrates the LFNG antibody detected the LFNG protein (arrow).
Application
LFNG Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LFNG Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
LFNG Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
