Application
LGTN Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the LGTN antibody detected the LGTN protein (arrow).Application
LGTN Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LGTN Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of LGTN Antibody (N-term)(Cat#AP12096a) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | LGTN Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF2D; HCA56; LGTN; Eukaryotic translation initiation factor 2D; Hepatocellular carcinoma-associated antigen 56; Ligatin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HCA56 LGTN |
|---|---|
| Alternative Names | EIF2D HCA56 LGTN Eukaryotic translation initiation factor 2D Hepatocellular carcinoma-associated antigen 56 Ligatin |
| Molecular Weight(MW) | 64706 Da |
| Function | Translation initiation factor that is able to deliver tRNA to the P-site of the eukaryotic ribosome in a GTP-independent manner. The binding of Met-tRNA(I) occurs after the AUG codon finds its position in the P-site of 40S ribosomes, the situation that takes place during initiation complex formation on some specific RNAs. Its activity in tRNA binding with 40S subunits does not require the presence of the aminoacyl moiety. Possesses the unique ability to deliver non-Met (elongator) tRNAs into the P- site of the 40S subunit. In addition to its role in initiation, can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits |
| Tissue Specificity | This LGTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-33 amino acids from the N-terminal region of human LGTN. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 1939 |
|---|---|
| Nucleotide Accession | NP_008824.2 |
Application
LGTN Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the LGTN antibody detected the LGTN protein (arrow).
Application
LGTN Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LGTN Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of LGTN Antibody (N-term)(Cat#AP12096a) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
