Application
Western blot analysis of LIF Antibody (Center) in CEM cell line lysates (35ug/lane). LIF (arrow) was detected using the purified Pab.Application
Western blot analysis of LIF (arrow) using rabbit polyclonal LIF Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LIF gene.Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with LIF Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of CEM cells using LIF Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | LIF Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LIF; HILDA; Leukemia inhibitory factor; Differentiation-stimulating factor; Melanoma-derived LPL inhibitor; INN=Emfilermin; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HILDA |
|---|---|
| Alternative Names | LIF HILDA Leukemia inhibitory factor Differentiation-stimulating factor Melanoma-derived LPL inhibitor INN=Emfilermin Flags: Precursor |
| Molecular Weight(MW) | 22008 Da |
| Function | LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes |
| Tissue Specificity | This LIF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79~109 amino acids from the Center region of human LIF. |
| Cellular Localization | Secreted. |
| Entrez Gene | 3976 |
|---|
Application
Western blot analysis of LIF Antibody (Center) in CEM cell line lysates (35ug/lane). LIF (arrow) was detected using the purified Pab.
Application
Western blot analysis of LIF (arrow) using rabbit polyclonal LIF Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LIF gene.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with LIF Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of CEM cells using LIF Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
