Application
LIMA1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the LIMA1 antibody detected the LIMA1 protein (arrow).| Product Name | LIMA1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LIMA1; EPLIN; SREBP3; LIM domain and actin-binding protein 1; Epithelial protein lost in neoplasm |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EPLIN SREBP3 |
|---|---|
| Alternative Names | LIMA1 EPLIN SREBP3 LIM domain and actin-binding protein 1 Epithelial protein lost in neoplasm |
| Molecular Weight(MW) | 85226 Da |
| Function | Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments |
| Tissue Specificity | Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium |
| Cellular Localization | Cytoplasm. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Note=This cytoskeletal protein co- localizes with actin stress fibers and focal adhesion plaques |
| Entrez Gene | 51474 |
|---|---|
| Nucleotide Accession | NP_001107019.1 NP_001107018.1 |
Application
LIMA1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the LIMA1 antibody detected the LIMA1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
