Application
LNPEP Antibody (N-Term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the LNPEP antibody detected the LNPEP protein (arrow).Application
LNPEP antibody (N-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LNPEP antibody (N-Term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
LNPEP Antibody (N-Term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | LNPEP Antibody (N-Term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LNPEP; OTASE; Leucyl-cystinyl aminopeptidase; Insulin-regulated membrane aminopeptidase; Insulin-responsive aminopeptidase; Oxytocinase; Placental leucine aminopeptidase; Leucyl-cystinyl aminopeptidase, pregnancy serum form |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OTASE |
|---|---|
| Alternative Names | LNPEP OTASE Leucyl-cystinyl aminopeptidase Insulin-regulated membrane aminopeptidase Insulin-responsive aminopeptidase Oxytocinase Placental leucine aminopeptidase Leucyl-cystinyl aminopeptidase pregnancy serum form |
| Molecular Weight(MW) | 117349 Da |
| Function | Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain |
| Tissue Specificity | Highly expressed in placenta, heart, kidney and small intestine. Detected at lower levels in neuronal cells in the brain, in skeletal muscle, spleen, liver, testes and colon |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. Note=In brain only the membrane-bound form is found. The protein resides in intracellular vesicles together with GLUT4 and can then translocate to the cell surface in response to insulin and/or oxytocin. Localization may be determined by dileucine internalization motifs, and/or by interaction with tankyrases |
| Entrez Gene | 4012 |
|---|---|
| Nucleotide Accession | NP_005566.2 NP_787116.2 |
Application
LNPEP Antibody (N-Term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the LNPEP antibody detected the LNPEP protein (arrow).
Application
LNPEP antibody (N-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LNPEP antibody (N-Term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
LNPEP Antibody (N-Term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
