Application
Western blot analysis of LPIN2 Antibody (Center) in K562 cell line lysates (35ug/lane).LPIN2 (arrow) was detected using the purified Pab.(2ug/ml)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with LPIN2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of K562 cells using LPIN2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | LPIN2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0249 |
|---|---|
| Alternative Names | LPIN2 KIAA0249 Phosphatidate phosphatase LPIN2 Lipin-2 |
| Molecular Weight(MW) | 99399 Da |
| Function | Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A to modulate lipid metabolism (By similarity) |
| Tissue Specificity | Expressed in liver, lung, kidney, placenta, spleen, thymus, lymph node, prostate, testes, small intestine, and colon |
| Cellular Localization | Nucleus (By similarity). Cytoplasm, cytosol (By similarity). Endoplasmic reticulum membrane (By similarity) Note=Translocates to endoplasmic reticulum membrane with increasing levels of oleate (By similarity) |
| Entrez Gene | 9663 |
|---|
Application
Western blot analysis of LPIN2 Antibody (Center) in K562 cell line lysates (35ug/lane).LPIN2 (arrow) was detected using the purified Pab.(2ug/ml)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with LPIN2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of K562 cells using LPIN2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
