Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-LRP6 (pS1490) RabMAb (Cat. #AJ1449a), 1:1000 dilution. Cells were either (A) untreated (B) treated with Calyculin| Product Name | LRP6 Antibody Phospho (pS1490) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S1490 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2360Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Molecular Weight(MW) | 180429 Da |
|---|---|
| Function | Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity) |
| Tissue Specificity | Widely coexpressed with LRP5 during embryogenesis and in adult tissues |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Note=On Wnt signaling, undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location. Released from the endoplasmic reticulum on palmitoylation. Mono-ubiquitination retains it in the endoplasmic reticulum in the absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes with AXIN1 and GSK3B at the plasma membrane in LRP6-signalsomes. Chaperoned to the plasma membrane by MESD (By similarity) |
| Entrez Gene | 4040 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-LRP6 (pS1490) RabMAb (Cat. #AJ1449a), 1:1000 dilution. Cells were either (A) untreated (B) treated with Calyculin| Application Notes | WB:1:500~1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
