Application
Western blot analysis of RT25 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). RT25 (arrow) was detected using the purified Pab.Application
RT25 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RT25 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
RT25 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RT25 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RPMS25 |
|---|---|
| Alternative Names | MRPS25 RPMS25 28S ribosomal protein S25 mitochondrial |
| Molecular Weight(MW) | 20116 Da |
| Tissue Specificity | This RT25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144~173 amino acids from the C-terminal region of human RT25. |
| Cellular Localization | Mitochondrion. |
| Entrez Gene | 64432 |
|---|
Application
Western blot analysis of RT25 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). RT25 (arrow) was detected using the purified Pab.
Application
RT25 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RT25 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
RT25 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
