| Product Name | 14-3-3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3F7] to 14 - 3 - 3 - BSA and Azide freeMembers of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf-1 kinase activity and phosphorylation amoung many other functions. |
| Immunogen | Recombinant full length protein (Human 14-3-3 gamma). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3F7 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 mg/ml. Predicted molecular weight: 30 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | 1433 |
|---|---|
| Target | 14-3-3 |
| Tissue Specificity | Recognizes human beta, gamma, sigma and zeta isoforms of 14-3-3. |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
| Positive Control | HeLa whole cell lysate |
|---|---|
| Application Notes | IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 mg/ml. Predicted molecular weight: 30 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: PBS. pH 7.4 |
