Application
Western blots of 14-3-3 isoforms in HEK293 cell extracts using GeneTex antibodies. Lane 1 = anti-gamma 14-3-3 Lane 2 = anti-14-3-3 zeta (acetyl) antibody Lane 3 = anti-14-3-3 epsilon antibody, C-term Lane 4 = anti-14-3-3 epsilon (acetyl) antibody, N-term Lane 5 = anti-14-3-3 eta (acetyl) antibody The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 which migrates at 33kDa .| Product Name | 14-3-3 epsilon antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to 14-3-3 epsilon |
| Immunogen | Synthetic corresponding to C-terminal sequence of sheep 14-3-3 epsilon.Peptide sequence: CGEEQNKEALQDVEDENQ |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| Western Blotting: Max Dilution 1/3000 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | HumanMouseChickenRatRabbitSheepBovine |
| Predicted Reactivity | Mammal |
| Concentration | 1mg/ml |
| Purification | Serum |
| Target | 14-3-3 Epsilon |
|---|---|
| Tissue Specificity | This antibody recognizes a C-terminal epitope of 14-3-3 epsilon. |
Application
Western blots of 14-3-3 isoforms in HEK293 cell extracts using GeneTex antibodies. Lane 1 = anti-gamma 14-3-3 Lane 2 = anti-14-3-3 zeta (acetyl) antibody Lane 3 = anti-14-3-3 epsilon antibody, C-term Lane 4 = anti-14-3-3 epsilon (acetyl) antibody, N-term Lane 5 = anti-14-3-3 eta (acetyl) antibody The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 which migrates at 33kDa .| Application Notes | Western Blotting: Max Dilution 1/3000 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | 0.09%Sodium Azide (NaN3) |
