| Product Name | 14-3-3 gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3F8] to 14-3-3 gammaMembers of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. 14-3-3 proteins regulate many cellular processes that are important in cancer biology, such as apoptosis and cell cycle checkpoints. Evidence indicates 14-3-3 is required for Raf1 kinase activity and phosphorylation amoung many other functions. Levels of 14-3-3 gamma in the brain are significantly increased in Alzheimer's disease and Down syndrome. |
| Immunogen | Recombinant full length protein (Human 14-3-3 gamma). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3F8 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 30 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | 1433 gamma 7532 14-3-3GAMMA 1433 P61981 YWHAG 1433GAMMA 605356 |
|---|---|
| Target | 14-3-3 gamma |
| Tissue Specificity | No crossreactivity with other isoforms of 14-3-3. |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
| Positive Control | HeLa whole cell lysate |
|---|---|
| Application Notes | IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 30 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC. For extended storage add 50% glycerol, aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: 10mM PBS |
