Application
Western Blot: 14-3-3 gamma Antibody (HS23) [ - 14-3-3 gamma detected in lysates using Lane 1: HeLa cell lysate, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).Application
Immunocytochemistry/Immunofluorescence: 14-3-3 gamma Antibody (HS23) [ - Detection of 14-3-3 gamma (green) in HepG2 cells using Nuclei (Blue) are counterstained with Hoechst 33258| Product Name | 14-3-3 gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal to 14-3-3 gamma (clone HS23)14-3-3 proteins are present as multigene families in most organisms. Signal-induced phosphorylation has the ability to change protein function. Sometimes, however, phosphorylation is not enough to change a proteinis function. 14-3-3 proteins play an important role of bringing signal transduction to completion. They regulate many cellular processes that are important in cancer biology, such as apoptosis and cell-cycle checkpoints. |
| Immunogen | This antibody was raised against an N-terminal fragment of human 14-3-3 gamma. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | HS23 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| This antibody can be used in Western Blot where a distinct band around 33 kDa is detected.Suggested working dilutions: *Western Blot - 1:1,000*The investigator should determine the optimal working dilution for a specific application.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | 1433 gamma 7532 14-3-3GAMMA 1433 P61981 YWHAG 1433GAMMA 605356 |
|---|---|
| Target | 14-3-3 gamma |
| Tissue Specificity | This antibody is specific for human 14-3-3 gamma. |
Application
Western Blot: 14-3-3 gamma Antibody (HS23) [ - 14-3-3 gamma detected in lysates using Lane 1: HeLa cell lysate, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).
Application
Immunocytochemistry/Immunofluorescence: 14-3-3 gamma Antibody (HS23) [ - Detection of 14-3-3 gamma (green) in HepG2 cells using Nuclei (Blue) are counterstained with Hoechst 33258| Positive Control | HeLa whole cell lysate |
|---|---|
| Application Notes | This antibody can be used in Western Blot where a distinct band around 33 kDa is detected.Suggested working dilutions: *Western Blot - 1:1,000*The investigator should determine the optimal working dilution for a specific application.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4 degrees Celcius. Aliquot and store at -20 degrees Celcius for long-term storage. Avoid repeated freeze-thaws. |
| Storage Buffer | 0.1 ml of raw mouse ascites [6.8 mg/ml], containing 0.1% Sodium Azide. |
