Application
Immunofluorescence staining of methanol-fixed Hela cells using 14-3-3 zeta (Phospho-Ser58) Antibody .Application
Western blot analysis of extracts from Hela cells untreated or treated with UV using 14-3-3 zeta (Phospho-Ser58) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (Phospho-Ser58) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | 14-3-3 zeta (phospho Ser58) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 14-3-3 zeta (Phospho Ser58)This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. |
| Immunogen | Peptide sequence around phosphorylation site of serine 58 (R-S-S(p)-W-R) derived from Human 14-3-3 zeta. |
| Modification | p-Ser58 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 14-3-3-zeta KCIP-1 MGC111427 MGC126532 MGC138156 YWHAD YWHAZ phospholipase A2 14-3-3 zeta protein kinase C inhibitor protein-1 14-3-3 delta 14-3-3 protein zeta/delta 14-3-3 protein/cytosolic phospholipase A2 protein kinase C inhibitor protein |
|---|---|
| Target | 14-3-3 zeta (Phospho Ser58) |
| Molecular Weight(MW) | 28 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using 14-3-3 zeta (Phospho-Ser58) Antibody .
Application
Western blot analysis of extracts from Hela cells untreated or treated with UV using 14-3-3 zeta (Phospho-Ser58) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (Phospho-Ser58) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
