Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 12% SDS PAGE diluted at 1:1000| Product Name | 14-3-3 zeta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 14-3-3 zeta (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide)This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human 14-3-3 zeta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Xenopus laevis, Pig, Chicken, Sheep, Rhesus Monkey, BovineHuman (100%), Rat (99%), Xenopus laevis (85%), Pig (100%), Chicken (98%), Sheep (99%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 14-3-3-zeta antibody KCIP-1 antibody MGC111427 antibody MGC126532 antibody MGC138156 antibody YWHAD antibody YWHAZ antibody phospholipase A2 antibody 14-3-3 zeta antibody protein kinase C inhibitor protein-1 antibody 14-3-3 delta antibody 14-3-3 |
|---|---|
| Molecular Weight(MW) | 28 kDa (note) (7) |
| Cellular Localization | Cytoplasm , Melanosome |
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 12% SDS PAGE diluted at 1:1000| Positive Control | NIH-3T3 , JC |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
