Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample(30 µg of whole cell lysate) A:Hep G2 B:MOLT4 12% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLaS3, using 14-3-3 zeta antibody at 1:100 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using 14-3-3 zeta antibody at 1:100 dilution.Application
14-3-3 zeta antibody detects 14-3-3 zeta protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. 14-3-3 zeta antibody dilution: 1:500.| Product Name | 14-3-3 zeta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 14-3-3 zeta (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide)This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human 14-3-3 zeta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Chicken, Sheep, Rhesus Monkey, BovineRat (99%), Zebrafish (80%), Xenopus laevis (89%), Pig (99%), Chicken (99%), Sheep (99%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 14-3-3-zeta antibody KCIP-1 antibody MGC111427 antibody MGC126532 antibody MGC138156 antibody YWHAD antibody YWHAZ antibody phospholipase A2 antibody 14-3-3 zeta antibody protein kinase C inhibitor protein-1 antibody 14-3-3 delta antibody 14-3-3 |
|---|---|
| Molecular Weight(MW) | 28 kDa (note) (6) |
| Cellular Localization | Cytoplasm , Melanosome |
Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample(30 µg of whole cell lysate) A:Hep G2 B:MOLT4 12% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLaS3, using 14-3-3 zeta antibody at 1:100 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using 14-3-3 zeta antibody at 1:100 dilution.
Application
14-3-3 zeta antibody detects 14-3-3 zeta protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. 14-3-3 zeta antibody dilution: 1:500.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
