Application
WB ImageWestern blots of 14-3-3 isoforms in HEK293 cell extracts using GeneTex antibodies. Lane 1 = anti-gamma 14-3-3 Lane 2 = anti-zeta 14-3-3 Lane 3 = anti-C-epsilon-14-3-3 Lane 4 = anti-N-epsilon-14-3-3 Lane 5 = anti-eta 14-3-3. The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 which migrates at 33kDa| Product Name | 14-3-3 zeta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to 14-3-3 zeta |
| Immunogen | Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 zeta/delta.Peptide sequence: Ac.MDKNELVQKAC |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| Western Blotting: Max Dilution 1/3000: |
|
| Species Reactivity | HumanMouseChickenRatRabbitSheepBovine |
| Predicted Reactivity | Mammal |
| Concentration | 1mg/ml |
| Purification | Serum |
| Gene Synonyms | acetyl 14-3-3 zeta 14-3-3 zeta acetyl 14-3-3 zeta acetyl 14-3-3 zeta acetyl acetyl 14-3-3 zeta acetyl 14-3-3 zeta |
|---|---|
| Target | 14-3-3 zeta/Delta (acetyl) |
| Tissue Specificity | This antibody recognizes the acetylated N-terminal of 14-3-3 zeta and the phosphorylated zeta isoform (also known as 14-3-3 delta) in all mammals. It may not react with recombinant proteins that are not N-acetylated. |
Application
WB ImageWestern blots of 14-3-3 isoforms in HEK293 cell extracts using GeneTex antibodies. Lane 1 = anti-gamma 14-3-3 Lane 2 = anti-zeta 14-3-3 Lane 3 = anti-C-epsilon-14-3-3 Lane 4 = anti-N-epsilon-14-3-3 Lane 5 = anti-eta 14-3-3. The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3 which migrates at 33kDa| Application Notes | Western Blotting: Max Dilution 1/3000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | 0.09%Sodium Azide (NaN3) |
