Application
Untreated (–) and treated (+) 293T whole cell extracts (60 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Ser65) antibody diluted at 1:500.Application
Wild-type (WT) and 4E-BP1 (phospho Ser65) knockout (KO) HeLa cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Ser65) antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.| Product Name | 4E-BP1 (phospho Ser65) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 4E-BP1 (phospho Ser65) (eukaryotic translation initiation factor 4E binding protein 1)This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human 4E-BP1 (phospho Ser65). The exact sequence is proprietary. |
| Modification | p-Ser65 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Pig, BovinePig (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | 4E-BP1 (phospho Ser65) |
|---|---|
| Molecular Weight(MW) | 13 kDa (note) |
Application
Untreated (–) and treated (+) 293T whole cell extracts (60 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Ser65) antibody diluted at 1:500.
Application
Wild-type (WT) and 4E-BP1 (phospho Ser65) knockout (KO) HeLa cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Ser65) antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.| Positive Control | 293T (starvation for 24 hr) , 293T (starvation for 24 hr and 20%FBS treatment for 0.5 hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
