Application
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term diluted at 1:500.Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term diluted at 1:500.Application
53BP1 antibody [N1], N-term detects TP53BP1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract 5 % SDS-PAGE 53BP1 antibody [N1], N-term dilution: 1:500Application
53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 53BP1 protein stained by 53BP1 antibody [N1], N-term diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.Application
53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. 53BP1 antibody [N1], N-term dilution: 1:250.| Product Name | 53BP1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 53BP1 (tumor protein p53 binding protein 1)May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human 53BP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 53BP1 antibody FLJ41424 antibody MGC138366 antibody p202 antibody TP53BP1 antibody tumor suppressor p53-binding protein 1 antibody tumor protein 53-binding protein 1 antibody tumor protein p53-binding protein 1 antibody p53BP1 antibody p53-bindi |
|---|---|
| Molecular Weight(MW) | 214 kDa (note) (3) |
| Cellular Localization | Nucleus , Kinetochore |
Application
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term diluted at 1:500.
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term diluted at 1:500.
Application
53BP1 antibody [N1], N-term detects TP53BP1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract 5 % SDS-PAGE 53BP1 antibody [N1], N-term dilution: 1:500
Application
53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 53BP1 protein stained by 53BP1 antibody [N1], N-term diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.
Application
53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. 53BP1 antibody [N1], N-term dilution: 1:250.| Positive Control | 293T , HeLa , Neuro 2A , C8D30 , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
