Application
Dot blot analysis of synthetic oligonucleotides containing different modified C-bases using 5-Carboxylcytosine antibody at a dilution of 1:1,000. A 125 and 25 ng of the respective oligo’s were bound to a Streptavindin-coated multi-well plate. The The binding of antibody to the DNA was measured by ECL chemiluminescence.Application
Immunoprecipitation was performed with on 2 μg of J1 ES genomic DNA, spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IP’d DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control.Application
ICC/IF analysis of 293T cells transfected with either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD) using 5-Carboxylcytosine antibody (red) at a dilution of 1:500, and an anti-FLAG antibody (green), followed by DAPI counterstaining (blue).| Product Name | 5-Carboxylcytosine / 5-caC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to 5-caC |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotICC/IFIP |
| ICC/IF:1:200~1:500 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | 5-caC |
|---|
Application
Dot blot analysis of synthetic oligonucleotides containing different modified C-bases using 5-Carboxylcytosine antibody at a dilution of 1:1,000. A 125 and 25 ng of the respective oligo’s were bound to a Streptavindin-coated multi-well plate. The The binding of antibody to the DNA was measured by ECL chemiluminescence.
Application
Immunoprecipitation was performed with on 2 μg of J1 ES genomic DNA, spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IP’d DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control.
Application
ICC/IF analysis of 293T cells transfected with either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD) using 5-Carboxylcytosine antibody (red) at a dilution of 1:500, and an anti-FLAG antibody (green), followed by DAPI counterstaining (blue).| Application Notes | ICC/IF:1:200~1:500 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | preservative buffer (PBS (pH 7.4), 30% glycerol, 0.05% sodium azide). |
