Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2B Receptor antibody diluted at 1:1000.
Application
Boiled and unboiled SH-SY5Y cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2B Receptor antibody diluted at 1:1000. (WCE: whole cell extract; ME: membrane extract)