Rabbit polyclonal to 5-HT3A receptorSerotonin (5-hydroxytryptamine, 5HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems. The 5HT3 receptors are distinguished from other 5HT receptors by their structure and function. 5HT3 receptors span the membrane only four times and are ligand-gated, cation-selective ion channels that mediate neuronal excitation and exocytotic release of neurotransmitters within the peripheral and central nervous systems.
Immunogen
Synthetic peptides, corresponding to amino acids 21-36 and 421-436 of Rat 5HT3 Receptor.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHCWB
For WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 47-50 kDa. Optimal dilutions/concentrations should be determined by the researcher.:
IHC analysis of 5-HT3R in human testis using 5-HT3R antibody at 5 ug/ml.
Application
Western blot analysis of 5-HT3 Receptor in A) human, B) mouse and C) rat brain tissue lysate using 5-HT3 Receptor antibody at 3 ug/ml.
Positive Control
Tested with human brain tissue.
Application Notes
For WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 47-50 kDa. Optimal dilutions/concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4oC short term. For extended storage aliquot and store at -20oC or below. Avoid freeze-thaw cycles.