Application
Western Blot: SR-5B Antibody [ - Western blot (WB) analysis of SR-5B (V300) antibody in extracts from COLO cells.| Product Name | 5-HT5B receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 5-HT5B receptorSR-5B, also designated 5-hydroxytryptamine (serotonin) receptor 5B (5-HT5B) and G protein-coupled receptor 134 (GPR134), is a receptor for the monoamine ligand serotonin (5-hydroxytryptamine, 5-HT). Serotonin is a neurotransmitter derived from serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin actions are mediated by receptors that influence the biochemistry of depression, anxiety, sexuality and appetite. Rat SR-5B is present in serotonergic neurons in dorsal raphe (DR) and central superior nucleus (CS, median raphe nucleus). DR cell bodies showing SR-5B mRNA expression are abundant in the medial portions of the nucleus. CS coexpression of SR-5B receptor mRNA with serotonin transporter mRNA is high in the intermediate portions of the nucleus. Serotonin receptors include SR-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| We recommend the following starting dilutions: ELISA 1:10000-1:20000, Western Blot 1:500-1:1000,. Optimal concentration should be determined experimentally by the end user.: |
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| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 645694 SerotoninNAcetyltransferase Arylalkamine NAcetyltransferase Serotonin-N-Acetyltransferase Arylalkamine N-Acetyltransferase HTR5B SNAT Serotonin N Acetyltransferase |
|---|---|
| Target | 5-HT5B receptor |
| Tissue Specificity | SR-5B (V300) detects endogenous levels of SR-5B protein. |
Application
Western Blot: SR-5B Antibody [ - Western blot (WB) analysis of SR-5B (V300) antibody in extracts from COLO cells.| Application Notes | We recommend the following starting dilutions: ELISA 1:10000-1:20000, Western Blot 1:500-1:1000,. Optimal concentration should be determined experimentally by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline, pH 7.2. |
