Application
ELISA was performed using a serial dilution of 5-Hydroxymethylcytosine antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1: 3,500.Application
5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade detects 5-Hydroxymethylcytosine / 5-hmC protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. 5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade dilution: 1:500.Application
5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade detects 5-Hydroxymethylcytosine / 5-hmC protein at nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. 5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade dilution: 1:500.Application
5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade detects 5-Hydroxymethylcytosine / 5-hmC protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. 5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade dilution: 1:500.| Product Name | 5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to 5-hmC |
| Immunogen | Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAIHC-PIPHMeDIP |
| hMeDIP* (3.5 µg/IP), ELISA (1:100 - 1:500),IHC-P (1:100 - 1:1000), Dot blotting (1:1,000), *Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | 5-hmC |
|---|
Application
ELISA was performed using a serial dilution of 5-Hydroxymethylcytosine antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1: 3,500.
Application
5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade detects 5-Hydroxymethylcytosine / 5-hmC protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. 5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade dilution: 1:500.
Application
5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade detects 5-Hydroxymethylcytosine / 5-hmC protein at nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. 5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade dilution: 1:500.
Application
5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade detects 5-Hydroxymethylcytosine / 5-hmC protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. 5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade dilution: 1:500.| Application Notes | hMeDIP* (3.5 µg/IP), ELISA (1:100 - 1:500),IHC-P (1:100 - 1:1000), Dot blotting (1:1,000), *Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
