Application
Western blot of rat hippocampal lysate showing phospho-specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser 523 using 5-Lipoxygenase (phospho Ser523) antibody .| Product Name | 5-Lipoxygenase (phospho Ser523) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to 5-Lipoxygenase (phospho Ser523)Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes. |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser523 conjugated to KLH |
| Modification | p-Ser523 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LOG5 5-LO 5LPG 5LO ALOX5 5 Lipoxygenase 5Lipoxygenase 5 LO 5-Lipoxygenase (phospho S523) 5-Lipoxygenase (phospho Ser523) 5-Lipoxygenase (pS523) 5-Lipoxygenase (pSer523) 5-Lipoxygenase phospho S523 5-Lipoxygenase phospho Ser523 Phospho 5-Lipox |
|---|---|
| Target | 5-Lipoxygenase (phospho Ser523) |
| Molecular Weight(MW) | 80 (note) |
| Cellular Localization | Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association. |
Application
Western blot of rat hippocampal lysate showing phospho-specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser 523 using 5-Lipoxygenase (phospho Ser523) antibody .| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
