Application
Immunocytochemical staining of HeLa cells using rabbit monoclonal anti-5-mC (clone RM231) antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).Application
ELISA of single stranded DNA using rabbit monoclonal anti-5-mC (clone RM231) antibody. The plate was coatedwith streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of RM231 was used as the primary antibody, and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Application
Dot blot of double stranded DNA using rabbit monoclonal anti-5-mC (clone RM231) antibody. The membrane waspre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with RM231.| Product Name | 5-Methylcytosine / 5-mC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM231] to 5-Methylcytosine / 5-mC |
| Immunogen | BSA-conjugated 5-methylcytosine. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM231 |
| Host Species | Rabbit |
| Tested Applications | DotELISAICC/IFIHCMeDIP |
| Dot Blot: 0.5 ug/mL – 2 ug/mL, ELISA: 0.1 ug/mL - 1 ug/mL, ICC: 0.5 ug/mL - 2 ug/mL.: |
|
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | 5-Methylcytosine / 5-mC |
|---|---|
| Tissue Specificity | This antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA. |
Application
Immunocytochemical staining of HeLa cells using rabbit monoclonal anti-5-mC (clone RM231) antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).
Application
ELISA of single stranded DNA using rabbit monoclonal anti-5-mC (clone RM231) antibody. The plate was coatedwith streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of RM231 was used as the primary antibody, and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Application
Dot blot of double stranded DNA using rabbit monoclonal anti-5-mC (clone RM231) antibody. The membrane waspre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with RM231.| Application Notes | Dot Blot: 0.5 ug/mL – 2 ug/mL, ELISA: 0.1 ug/mL - 1 ug/mL, ICC: 0.5 ug/mL - 2 ug/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
