Application
Flow Cytometry using ABCB5 Antibody (N-Term) on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit as a secondary antibody. Analysis was done on an FC500 flow cytometerApplication
Western blot of K562 cell line lysates (35 ug/lane).Application
Human Breast (formalin-fixed, paraffin-embedded) stained with ABCB5 antibody followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.Application
Flow Cytometry using ABCB5 Antibody (N-Term) on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit as a secondary antibody. Analysis was done on an FC500 flow cytometer.| Product Name | ABCB5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ABCB5ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM] |
| Immunogen | KLH conjugated synthetic peptide selected from the N-terminal region of human ABCB5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAFACSIHC-PWB |
| WB (1:50 - 1:100), Flo, ELISA (1:1000), IHC-P (10 µg/ml): |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ABCB5alpha ABCB5beta EST422562 |
|---|
Application
Flow Cytometry using ABCB5 Antibody (N-Term) on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit as a secondary antibody. Analysis was done on an FC500 flow cytometer
Application
Western blot of K562 cell line lysates (35 ug/lane).
Application
Human Breast (formalin-fixed, paraffin-embedded) stained with ABCB5 antibody followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
Application
Flow Cytometry using ABCB5 Antibody (N-Term) on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit as a secondary antibody. Analysis was done on an FC500 flow cytometer.| Application Notes | WB (1:50 - 1:100), Flo, ELISA (1:1000), IHC-P (10 µg/ml): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Storage Buffer | PBS, 0.09% sodium azide |
