Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:1000Application
ABCD4 antibody detects ABCD4 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: ABCD4 protein stained by ABCD4 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded AGS xenograft, using ABCD4 antibody at 1:500 dilution.| Product Name | ABCD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ABCD4 (ATP-binding cassette, sub-family D (ALD), member 4)The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. The function of this peroxisomal membrane protein is unknown. However, it is speculated that it may function as a heterodimer for another peroxisomal ABC transporter and, therefore, may modify the adrenoleukodystrophy phenotype. It may also play a role in the process of peroxisome biogenesis. Alternative splicing results in at least two different transcript variants, one which is protein-coding and one which is probably not protein-coding. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human ABCD4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (91%), Rat (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ABC41 antibody EST352188 antibody P70R antibody P79R antibody PMP69 antibody PXMP1L antibody ABCD4 antibody PXMP1-L antibody ATP-binding cassette sub-family D member 4 antibody 69 kDa peroxisomal ABC-transporter antibody PMP70-related protein ant |
|---|---|
| Molecular Weight(MW) | 69 kDa (note) (2) |
| Cellular Localization | Peroxisome membrane |
Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:1000
Application
ABCD4 antibody detects ABCD4 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: ABCD4 protein stained by ABCD4 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded AGS xenograft, using ABCD4 antibody at 1:500 dilution.| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
