Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ABCG2 antibody detects ABCG2 protein by western blot analysis. A. 30 µg A549 whole cell lysate/extract B. 30 µg H1299 whole cell lysate/extract C. 30 µg HCT116 whole cell lysate/extract 7.5% SDS-PAGE ABCG2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ABCG2 antibody detects ABCG2 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: ABCG2 antibody dilution: 1:250.| Product Name | ABCG2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ABCG2 (ATP-binding cassette, sub-family G (WHITE), member 2)The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ABCG2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Sheep, Rhesus Monkey, BovineRat (82%), Dog (83%), Pig (86%), Sheep (86%), Rhesus Monkey (97%), Bovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ABC15 antibody ABCP antibody BCRP antibody BCRP1 antibody BMDP antibody CD338 antibody CDw338 antibody EST157481 antibody MGC102821 antibody MRX antibody MXR antibody MXR1 antibody ABCG2 antibody ATP-binding cassette sub-family G member 2 anti |
|---|---|
| Molecular Weight(MW) | 72 kDa (note) (2) |
| Cellular Localization | Cell membrane |
Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ABCG2 antibody detects ABCG2 protein by western blot analysis. A. 30 µg A549 whole cell lysate/extract B. 30 µg H1299 whole cell lysate/extract C. 30 µg HCT116 whole cell lysate/extract 7.5% SDS-PAGE ABCG2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ABCG2 antibody detects ABCG2 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: ABCG2 antibody dilution: 1:250.| Positive Control | A549 , H1299 , HCT116 , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
