Application
Immunofluorescent staining of HeLa cells using anti-ACAT2 mouse monoclonal antibody .Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2.Application
Anti-ACAT2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with ACAT2Application
HEK293T cells transfected with either RC201821 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACAT2 antibody, and then analyzed by flow cytometry.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.| Product Name | ACAT2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3A8] to ACAT2The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. [provided by RefSeq] |
| Immunogen | Full length human recombinant protein of human ACAT2 (NP_0058826) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 3A8 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 : |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | cytosolic acetoacetyl-CoA thiolase acetylCoenzyme A acetyltransferase2 acetoacetyl Coenzyme A thiolase OTTHUMP00000017527 acetylCoenzyme A acetyltransferase 2 acetyl-Coenzyme A acetyltransferase 2 ACAT2 cytosolic acetoacetylCoA thiolase |
|---|---|
| Target | ACAT2 |
| Tissue Specificity | is specific for human ACAT2 (NP_0058826). |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescent staining of HeLa cells using anti-ACAT2 mouse monoclonal antibody .
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2.
Application
Anti-ACAT2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with ACAT2
Application
HEK293T cells transfected with either RC201821 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACAT2 antibody, and then analyzed by flow cytometry.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.| Application Notes | ICC/IF 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
