Application
Clone RM101 recognizes acetylated lysine in peptides with different sequences. It does not react with non-acetylated lysine.Application
Immunocytochemistry of A431 cells nontreated or treated with Trichostatin A (TSA), using Clone RM101 at 1/500 dilution (followed by PE conjugated secondary antibody, red) and DAPI (blue).Application
Immunoprecipitation of A431 cell lysates, by clone RM101 at 1/500 dilution, was blotted with anti-PTEN mouse monoclonal antibody.Application
Western blot of HeLa cells nontreated or treated with Trichostatin A (TSA), using Clone RM101 at 1/2000 dilution.| Product Name | Acetylated-Lysine antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM101] to Acetylated-Lysine |
| Immunogen | Acetyllysine -BSA |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM101 |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIHCIPWB |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | All |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Acetylated-Lysine |
|---|---|
| Tissue Specificity | This antibody reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, and lysine with other modification. |
Application
Clone RM101 recognizes acetylated lysine in peptides with different sequences. It does not react with non-acetylated lysine.
Application
Immunocytochemistry of A431 cells nontreated or treated with Trichostatin A (TSA), using Clone RM101 at 1/500 dilution (followed by PE conjugated secondary antibody, red) and DAPI (blue).
Application
Immunoprecipitation of A431 cell lysates, by clone RM101 at 1/500 dilution, was blotted with anti-PTEN mouse monoclonal antibody.
Application
Western blot of HeLa cells nontreated or treated with Trichostatin A (TSA), using Clone RM101 at 1/2000 dilution.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
