| Product Name | Acidic Cytokeratin antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AE1] (ready-to-use) to Acidic CytokeratinThis gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008] |
| Immunogen | BALB/C mice were immunized with human epidermal keratin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AE1 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| This antibody has been pretitered and quality controlled to work allon formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. Proteolytic digestion of tissue sections is required prior to immunostaining. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KRT10 BIE K10 KPP EHK CK10 BCIE |
|---|---|
| Target | Acidic Cytokeratin |
| Tissue Specificity | This antibody recognizes most of the acidic (Type I) keratins. It recognizes keratin polypeptides of 50 and 56.5 kD. In keratinized epidermis, 50 kD keratin is present in the basal layer, while 56.5 kD keratin is present in suprabasal layers. This antibody can be used to stain distinct cell layers in an epithelial tissue and various neoplasms of epithelial origin. |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
| Positive Control | Skin |
|---|---|
| Application Notes | This antibody has been pretitered and quality controlled to work allon formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. Proteolytic digestion of tissue sections is required prior to immunostaining. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline containing sodium azide |
