Application
Western blot analysis of Acinus in K562 whole cell lysate with Acinus antibody (NP) at 1μg/ml.| Product Name | Acinus antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to AcinusChromatin condensation and nuclear fragmentation(CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase-activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE (1). A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS` (2). |
| Immunogen | Synthetic peptide - amino acids 994-1009 of human Acinus L, 236-251 of human Acinus S and 267-282 of human Acinus S’, which are identical to those of mouse Acinus. The selected antigenic sequence is located near the N-terminus of active peptide p17. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ACINUS ACIN1 KIAA0670 22985 Q9UKV3 ACN 604562 DKFZp667N107 |
|---|---|
| Target | Acinus |
| Cellular Localization | Nuclear |
Application
Western blot analysis of Acinus in K562 whole cell lysate with Acinus antibody (NP) at 1μg/ml.| Positive Control | K562 whole cell lysate |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 0.02% sodium azide |
