Application
IP with (1:500) using 250 µl of solubilized COS1 cells expressing constitutively active form of ACK1. Western blot with at 1:500. Apparent MW of ACK1 is 100 kDa.| Product Name | ACK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ACK1This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. |
| Immunogen | Synthetic peptide – Immunogen sequence information is commercially sensitive |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP: Use at a dilution of 1/200. WB: Use at a dilution of 1/500. Detects a band of approximately 120 kDa. The antibody also reacts to a 100 kDa protein in various breast cancer cell lines. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | p21cdc42Hs 10188 ACK Q07912 ACK1 TNK2 606994 FLJ44758 FLJ45547 |
|---|---|
| Target | ACK1 |
| Cellular Localization | Cell Membrane |
Application
IP with (1:500) using 250 µl of solubilized COS1 cells expressing constitutively active form of ACK1. Western blot with at 1:500. Apparent MW of ACK1 is 100 kDa.| Positive Control | Tested with breast cancer cell lines. |
|---|---|
| Application Notes | IP: Use at a dilution of 1/200. WB: Use at a dilution of 1/500. Detects a band of approximately 120 kDa. The antibody also reacts to a 100 kDa protein in various breast cancer cell lines. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
