Application
Sample(30 μg of whole cell lysate) A:Raji 7.5% SDS PAGE diluted at 1:500Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000Application
ACK1 antibody [N1N2], N-term detects TNK2 protein at cytoplasm on human lung papillory adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded lung papillory adenocarcinoma. ACK1 antibody [N1N2], N-term dilution: 1:500.| Product Name | ACK1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ACK1 (tyrosine kinase, non-receptor, 2)This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human ACK1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (96%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACK antibody ACK1 antibody FLJ44758 antibody FLJ45547 antibody p21cdc42Hs antibody TNK2 antibody tyrosine kinase non-receptor protein 2 antibody activated CDC42 kinase 1 antibody activated p21cdc42Hs kinase antibody activated Cdc42-associated kina |
|---|---|
| Molecular Weight(MW) | 115 kDa (note) (3) |
| Cellular Localization | Cell membrane |
Application
Sample(30 μg of whole cell lysate) A:Raji 7.5% SDS PAGE diluted at 1:500
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000
Application
ACK1 antibody [N1N2], N-term detects TNK2 protein at cytoplasm on human lung papillory adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded lung papillory adenocarcinoma. ACK1 antibody [N1N2], N-term dilution: 1:500.| Positive Control | Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
