Application
Aconitase 1 antibody detects Aconitase 1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg HeLa whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Aconitase 1 antibody dilution: 1:1000Application
Aconitase 1 antibody [N1C1] detects Aconitase 1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Aconitase 1 protein stained by Aconitase 1 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | Aconitase 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Aconitase 1 (aconitase 1, soluble)Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Aconitase 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Rabbit, Chicken, Rhesus Monkey, BovineMouse (93%), Rat (93%), Zebrafish (81%), Xenopus laevis (85%), Rabbit (94%), Chicken (85%), Rhesus Monkey (99%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ACONS antibody IREB1 antibody IREBP antibody IREBP1 antibody IRP1 antibody ACO1 antibody citrate hydro-lyase antibody iron regulatory protein 1 antibody iron-responsive element-binding protein 1 antibody iron-responsive element binding protein 1 a |
|---|---|
| Molecular Weight(MW) | 98 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Aconitase 1 antibody detects Aconitase 1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg HeLa whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Aconitase 1 antibody dilution: 1:1000
Application
Aconitase 1 antibody [N1C1] detects Aconitase 1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Aconitase 1 protein stained by Aconitase 1 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
