Application
WB analysis of MCF-7 cells using ACSL1 antibody at 0.2-1μg/ml.Application
ICC/IF analysis of THP-1 cells using ACSL1 antibody at 1:200. Green : ASCL1, Red : Bodipy (stain for netural lipids). THP-1 cells was treated with 20mM PMA for 24hrs and then activated by culturing in the presence of irradiated M. tuberculosis at 0.050 mg/ml. for 3 days. Cells were fixed with 4% PFA, washed and stained with primary and then secondary antibody in blocking buffer containing 5% human serum.| Product Name | ACSL1 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ACSL1The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013] |
| Immunogen | A synthetic peptide corresponding to a N-terminal region of Human ACSL1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Cow: 85%; Dog: 92%; Guinea Pig: 85%; Horse: 79%; Human: 100%; Mouse: 85%; Pig: 92%; Rabbit: 100%; Rat: 85%; Zebrafish: 77% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LACS2 ACSL1 LACS1 ACS1 FACL2 LACS FACL1 |
|---|---|
| Target | ACSL1 |
| Molecular Weight(MW) | 78 KDa (note) |
Application
WB analysis of MCF-7 cells using ACSL1 antibody at 0.2-1μg/ml.
Application
ICC/IF analysis of THP-1 cells using ACSL1 antibody at 1:200. Green : ASCL1, Red : Bodipy (stain for netural lipids). THP-1 cells was treated with 20mM PMA for 24hrs and then activated by culturing in the presence of irradiated M. tuberculosis at 0.050 mg/ml. for 3 days. Cells were fixed with 4% PFA, washed and stained with primary and then secondary antibody in blocking buffer containing 5% human serum.| Positive Control | MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
