WB
Western blot analysis of various lysates using ACSL1 Rabbit mAb at 1:16000 dilution incubatedat room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.WB
Western blot analysis of various lysates using ACSL1 Rabbit mAb at 1:16000 dilution incubatedat room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.IHC
Immunohistochemistry analysis of paraffinembedded Human kidney tissue using ACSL1Rabbit mAb at a dilution of 1:800(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse fat tissue using ACSL1Rabbit mAb at a dilution of 1:800(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat fat tissue using ACSL1 RabbitmAb at a dilution of 1:800 (40xlens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.IF-P
Confocal imaging of paraffin-embeddedHuman liver tissue using ACSL1 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.IF-P
Confocal imaging of paraffin-embedded Ratliver tissue using ACSL1 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.IF-P
Confocal imaging of paraffin-embeddedMouse liver tissue using ACSL1 Rabbit mAb( dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.| Product Name | ACSL1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:4000-1:30000 IHC:1:800-1:8000 IF-P:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACSL1 |
|---|---|
| Gene Synonyms | ACS1 LACS FACL1 FACL2 LACS1 LACS2 |
| Gene Full Name | acyl-CoA synthetase long chain family member 1 |
| Gene Summary | The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013] |
| Alternative Names | ACS1 LACS FACL1 FACL2 LACS1 LACS2 |
| Molecular Weight(MW) | 78kDa(Observed band size:78kDa) |
| Cellular Localization | Endoplasmic reticulum membrane, Microsome membrane, Mitochondrion outer membrane, Peroxisome membrane, Single-pass type III membrane protein. |
WB
Western blot analysis of various lysates using ACSL1 Rabbit mAb at 1:16000 dilution incubatedat room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
WB
Western blot analysis of various lysates using ACSL1 Rabbit mAb at 1:16000 dilution incubatedat room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
IHC
Immunohistochemistry analysis of paraffinembedded Human kidney tissue using ACSL1Rabbit mAb at a dilution of 1:800(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse fat tissue using ACSL1Rabbit mAb at a dilution of 1:800(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat fat tissue using ACSL1 RabbitmAb at a dilution of 1:800 (40xlens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embeddedHuman liver tissue using ACSL1 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.
IF-P
Confocal imaging of paraffin-embedded Ratliver tissue using ACSL1 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.
IF-P
Confocal imaging of paraffin-embeddedMouse liver tissue using ACSL1 Rabbit mAb( dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.| Application Notes | WB:1:4000-1:30000 IHC:1:800-1:8000 IF-P:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
