Application
Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:1000Application
ACTR1B antibody detects ACTR1B protein at cytoskeleton by immunofluorescent analysis. Sample: SKNSH cells were fixed in ice-cold MeOH for 5 min. Green: ACTR1B protein stained by ACTR1B antibody diluted at 1:1000. Blue: Hoechst 33342 staining.Application
ACTR1B antibody detects ACTR1B protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded ovarian carcinoma. ACTR1B antibody dilution: 1:500.| Product Name | ACTR1B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ACTR1B (ARP1 actin-related protein 1 homolog B, centractin beta (yeast))This gene encodes a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90% amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ACTR1B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (100%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARP1B antibody CTRN2 antibody PC3 antibody ACTR1B antibody centractin beta antibody actin-related protein 1B antibody beta-centractin antibody ARP1 actin-related protein 1 homolog B centractin beta (yeast) antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytoskeleton , Centrosome |
Application
Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:1000
Application
ACTR1B antibody detects ACTR1B protein at cytoskeleton by immunofluorescent analysis. Sample: SKNSH cells were fixed in ice-cold MeOH for 5 min. Green: ACTR1B protein stained by ACTR1B antibody diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
ACTR1B antibody detects ACTR1B protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded ovarian carcinoma. ACTR1B antibody dilution: 1:500.| Positive Control | IMR32 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
